Reduction of Shelf-life of Dairy Products by a Heat-stable Protease from Pseudomonas Fluorescens P26
نویسندگان
چکیده
منابع مشابه
Characterisation of the Thermostable Protease AprX in Strains of Pseudomonas Fluorescens and Impact on the Shelf-life of Dairy Products: Preliminary Results
Bacterial proteases are involved in food spoilage and shelf-life reduction. Among the bacterial proteases, a predominant role in spoilage of dairy products seems to be played by the thermostable metallo-protease AprX, which is produced by various strains of Pseudomonas fluorescens. Differences in AprX enzyme activity among different strains were highlighted, but the most proteolytic strains wer...
متن کاملCharacterization of a Protease from a Psychrotroph, Pseudomonas fluorescens 114.
A psychrotrophic bacterium isolated from river sediment was identified as Pseudomonas fluorescens 114. It grew at 0 degrees C and optimally at 20 degrees C. The bacterium produced a protease with a molecular weight of 47,000, which was stable in the pH range of 5 to 9 and worked optimally between pH 6.5 and 10. Activity was optimal at 35 degrees C and was lost immediately at 50 degrees C and af...
متن کاملQuantitative studies of heat-stable proteinase from Pseudomonas fluorescens P1 by the enzyme-linked immunosorbent assay.
Pseudomonas fluorescens P1 is a psychrotrophic bacterium isolated from milk. Proteinase P1, the main extracellular heat-stable proteinase fraction of P. fluorescens P1, has been purified to homogeneity. A procedure with a sandwich enzyme-linked immunosorbent assay, using microplates and alkaline phosphatase conjugate was shown to detect 0.25 ng of proteinase P1 in 1 ml of reconstituted skim mil...
متن کاملInfluence of Incubation Conditions on Biofilm Formation by Pseudomonas Fluorescens Isolated from Dairy Products and Dairy Manufacturing Plants
In this study, biofilm formation of 64 Pseudomonas fluorescens strains isolated from milk, dairy products and dairy plants was compared. The strains were grown on Tryptic Soy Broth supplemented with 0.2% of glucose, on polystyrene microplates at 10 and 30°C for 48 h. In general, 57/64 P. fluorescens strains formed biofilm, although with great variability at both tested temperatures. Moreover, o...
متن کاملUtilization of a Stable Isotope of Oxygen by Pseudomonas Fluorescens.
Oxygen utilization is defined in this investigation as the terminal use of oxygen in respiration, i.e., the formation of water. A culture of Pseudomonas fluorescens was allowed to respire in an atmosphere of O(18). The production of H(2)O(18) was measured during two test runs of 124 and 232 min each. During the first run, 0.505 mmole of H(2)O(18) was produced. The second run produced 0.460 mmol...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1973
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(73)85265-8